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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    WB analysis of mouse pancreas using 15836-1-AP (same clone as 15836-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    WB analysis of mouse pancreas using 15836-1-AP (same clone as 15836-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human placenta using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human placenta using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human liver using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human liver using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human spleen using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human spleen using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human lung using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human lung using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human kidney using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IHC staining of human kidney using 15836-1-AP (same clone as 15836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Carboxypeptidase A1 Polyclonal antibody (15836-1-AP)

    IF Staining of U2OS using 15836-1-AP (same clone as 15836-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Carboxypeptidase A1 antibody (15836-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

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Carboxypeptidase A1 Polyclonal antibody, PBS Only

Carboxypeptidase A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15836-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CPA1, EC:3.4.17.1, CPA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15836-1-PBS targets Carboxypeptidase A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase A1 fusion protein Ag8586 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase A1 (pancreatic)
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC005279
Gene Symbol Carboxypeptidase A1
Gene ID (NCBI) 1357
RRIDAB_2085586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15085
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.