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  • Featured Product
  • KD/KO Validated

Carboxypeptidase E Polyclonal antibody, PBS Only

Carboxypeptidase E Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13710-1-PBS

Synonyms

Prohormone-processing carboxypeptidase, EC:3.4.17.10, CPH, CPE, Carboxypeptidase H

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IP experiment of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of rat brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IF Staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IF Staining of HepG2 using 13710-1-AP (same clone as 13710-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13710-1-PBS targets Carboxypeptidase E in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase E fusion protein Ag4647 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase E
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 50 kDa, 52 kDa
GenBank accession numberBC033866
Gene symbol Carboxypeptidase E
Gene ID (NCBI) 1363
RRIDAB_2083913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.