• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

Carboxypeptidase E Polyclonal antibody, PBS Only

Carboxypeptidase E Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13710-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IP experiment of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IF Staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Carboxypeptidase E Polyclonal antibody (13710-1-AP)

    IF Staining of HepG2 using 13710-1-AP (same clone as 13710-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13710-1-PBS targets Carboxypeptidase E in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase E fusion protein Ag4647 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase E
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 50 kDa, 52 kDa
GenBank accession numberBC033866
Gene symbol Carboxypeptidase E
Gene ID (NCBI) 1363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.