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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cardiac Troponin I Monoclonal antibody, PBS Only

Cardiac Troponin I Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D5D6

Cat no : 66376-1-PBS

Synonyms

cTnI, TNNI3, 1D5D6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Human heart tissue and skeletal muscle tissue (negative control) were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Mouse heart tissue (6 ug/lane) was subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    WB analysis of rat heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Rat heart tissue were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin Iantibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (66376-1-Ig, Clone: 1D5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Cardiac Troponin I Monoclonal antibody (66376-1-Ig)

    IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66376-1-PBS targets Cardiac Troponin I in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cardiac Troponin I fusion protein Ag16758 相同性解析による交差性が予測される生物種
Full Name troponin I type 3 (cardiac)
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC096165
Gene symbol Cardiac Troponin I
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.