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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cas9 Polyclonal antibody

Cas9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

bacteria and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 26758-1-AP

Synonyms

Cas9, Cas9 7A9, CRISPR, CRISPR/Cas9, CRISPR-associated endonuclease Cas9, csn1, SpyCas9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Cas9 Polyclonal antibody (26758-1-AP)

    WB analysis of HeLa using 26758-1-AP

    HeLa cells transfected with CRISPR-Cas9 were subjected to SDS PAGE followed by western blot with 26758-1-AP (Cas9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Cas9 Polyclonal antibody (26758-1-AP)

    WB analysis of A431 using 26758-1-AP

    A431 cells transfected with CRISPR-Cas9 were subjected to SDS PAGE followed by western blot with 26758-1-AP (Cas9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26758-1-AP targets Cas9 in WB, IP, ELISA applications and shows reactivity with bacteria samples.

Tested Reactivity bacteria
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cas9 fusion protein Ag25281 相同性解析による交差性が予測される生物種
Full Name Cas9
Calculated molecular weight 160 kDa
GenBank accession numberNP_269215
Gene symbol
Gene ID (NCBI)
RRIDAB_2918109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. This antibody detects the sp Cas9.

Protocols

Product Specific Protocols
WB protocol for Cas9 antibody 26758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Nature

Enhancer release and retargeting activates disease-susceptibility genes.

Authors - Soohwan Oh
View Article
humanWB

Clin Epigenetics

E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis

Authors - Jingxuan Li
View Article