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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Casein Kinase 1 Epsilon Polyclonal antibody, PBS Only

Casein Kinase 1 Epsilon Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11230-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    WB analysis of A431 using 11230-1-AP (same clone as 11230-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    WB analysis of mouse brain using 11230-1-AP (same clone as 11230-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human breast cancer using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human breast cancer using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human ovary using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human ovary using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human heart using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human heart using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human kidney using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human kidney using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human testis using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Casein Kinase 1 Epsilon Polyclonal antibody (11230-1-AP)

    IHC staining of human testis using 11230-1-AP (same clone as 11230-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11230-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11230-1-PBS targets Casein Kinase 1 Epsilon in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Casein Kinase 1 Epsilon fusion protein Ag1742 相同性解析による交差性が予測される生物種
Full Name casein kinase 1, epsilon
Calculated molecular weight 47 kDa
Observed molecular weight 46-52 kDa
GenBank accession numberBC006490
Gene symbol CK1 epsilon
Gene ID (NCBI) 1454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CSNK1E(Casein kinase I isoform epsilon) is also named as CKIe, CKI-epsilon and belongs to the casein kinase I subfamily. It plays a significant role in delaying the negative feedback signal within the transcription-translation-based autoregulatory loop that composes the core of the circadian mechanism and makes an ideal target for pharmaceutical compounds influencing circadian rhythms, sleep, and jet lag, as well as other physiologic and metabolic processes under circadian regulation(PMID:10775102). It can be autophosphorylated(PMID:19690332).