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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Caspase 10 Recombinant antibody (85196-3-RR)

    WB analysis using 85196-3-RR (same clone as 85196-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Caspase 10 Recombinant antibody (85196-3-RR)

    WB analysis using 85196-3-RR (same clone as 85196-3-PBS)

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Caspase 10 Recombinant antibody (85196-3-RR)

    FC experiment of HepG2 using 85196-3-RR (same clone as 85196-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-3-RR, Clone:242353H6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85196-3-PBS in a different storage buffer formulation.

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Caspase 10 Recombinant antibody, PBS Only

Caspase 10 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 85196-3-PBS
Clone No.242353H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

CASP10, Caspase10, CASP-10, CASP 10, Apoptotic protease Mch-4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85196-3-PBS targets Caspase 10 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name caspase 10, apoptosis-related cysteine peptidase
Calculated molecular weight 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC042844
Gene Symbol Caspase 10
Gene ID (NCBI) 843
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ92851
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full length has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.