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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Caspase 2/p18 Polyclonal antibody

Caspase 2/p18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24777-1-AP

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Caspase 2/p18 Polyclonal antibody (24777-1-AP)

    WB analysis of HEK-293 using 24777-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Caspase 2/p18 Polyclonal antibody (24777-1-AP)

    WB analysis of mouse kidney using 24777-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Caspase 2/p18 Polyclonal antibody (24777-1-AP)

    WB analysis of Jurkat using 24777-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caspase 2/p18 Polyclonal antibody (24777-1-AP)

    IHC staining of human liver cancer using 24777-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caspase 2/p18 Polyclonal antibody (24777-1-AP)

    IHC staining of human liver cancer using 24777-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Caspase 2/p18 Polyclonal antibody (24777-1-AP)

    IHC staining of human lung cancer using 24777-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Caspase 2/p18 Polyclonal antibody (24777-1-AP)

    IHC staining of human lung cancer using 24777-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Caspase 2/p18 Polyclonal antibody (24777-1-AP)

    IF Staining of HEK-293 using 24777-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24777-1-AP is Caspase 2/p18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 2/p18 fusion protein Ag20141 相同性解析による交差性が予測される生物種
Full Name caspase 2, apoptosis-related cysteine peptidase
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight48-51 kDa, 32 kDa
GenBank accession numberBC002427
Gene symbol Caspase 2
Gene ID (NCBI) 835
RRIDAB_2879715
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

Protocols

Product Specific Protocols
WB protocol for Caspase 2/p18 antibody 24777-1-APDownload protocol
IHC protocol for Caspase 2/p18 antibody 24777-1-APDownload protocol
IF protocol for Caspase 2/p18 antibody 24777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols