cleaved-Caspase 3 Polyclonal antibody

Name: cleaved-Caspase 3 Polyclonal antibody
Brand: Proteintech
SKU: 25546-1-AP

cleaved-Caspase 3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

130

Review Product

Cat no : 25546-1-AP

Synonyms

cleaved-Caspase 3

Validation Data Gallery

Filter:

AllWBIPIHCIF

WB analysis of HepG2 using 25546-1-AP
Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 25546-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 25546-1-AP
Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (cleaved-caspase 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25546-1-AP
Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 25546-1-AP
IP result of anti-cleaved-Caspase 3 (IP:25546-1-AP, 4ug; Detection:25546-1-AP 1:300) with Jurkat cells lysate 3200ug.
IHC staining of human skeletal muscle using 25546-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 25546-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 25546-1-AP
Immunofluorescent analysis of HepG2 cells using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 25546-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Tunicamycin treated HepG2 cells, Tunicamycin treated HeLa cells, Staurosporine treated Jurkat cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 110 publications below
IHC	See 9 publications below
IF	See 4 publications below
FC	See 1 publications below

Product Information

25546-1-AP targets cleaved-Caspase 3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, hamster, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight	32 kDa
Observed molecular weight	17 kDa
GenBank accession number	NM_032991
Gene symbol	Caspase 3
Gene ID (NCBI)	836
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspase 3, also named CPP32, SCA-1, and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7, and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize active-caspase 3.

Protocols

Product Specific Protocols
WB protocol for cleaved-Caspase 3 antibody 25546-1-AP	Download protocol
IHC protocol for cleaved-Caspase 3 antibody 25546-1-AP	Download protocol
IF protocol for cleaved-Caspase 3 antibody 25546-1-AP	Download protocol
IP protocol for cleaved-Caspase 3 antibody 25546-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Nat Methods DART-seq: an antibody-free method for global m6A detection. Authors - Kate D Meyer View Article
human	WB	Autophagy Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. Authors - Qichao Huang View Article
mouse	WB	Diabetes MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca(2+)-dependent Antioxidant Response. Authors - Lele Ji View Article
		PLoS Biol The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53. Authors - Hongmei Liu View Article
human	WB	Biol Res MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma. Authors - Tingting Wu View Article
rat	WB	Oxid Med Cell Longev Classic Prescription, Kai-Xin-San, Ameliorates Alzheimer's Disease as an Effective Multitarget Treatment: From Neurotransmitter to Protein Signaling Pathway. Authors - Sirui Guo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

cleaved-Caspase 3 Polyclonal antibody

cleaved-Caspase 3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 25546-1-AP

WB analysis of HepG2 using 25546-1-AP

WB analysis of Jurkat using 25546-1-AP

WB analysis of HeLa using 25546-1-AP

IP experiment of Jurkat using 25546-1-AP

IHC staining of human skeletal muscle using 25546-1-AP

IHC staining of human skeletal muscle using 25546-1-AP

IF Staining of HepG2 using 25546-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

DART-seq: an antibody-free method for global m6A detection.

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca(2+)-dependent Antioxidant Response.

The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53.

MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma.

Classic Prescription, Kai-Xin-San, Ameliorates Alzheimer's Disease as an Effective Multitarget Treatment: From Neurotransmitter to Protein Signaling Pathway.