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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Caspase 3 Recombinant antibody, PBS Only

Caspase 3 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5G20

Cat no : 82202-1-PBS

Synonyms

CASP3, Caspase3, Caspase-3, CASP-3, CASP 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using Caspase 3 Recombinant antibody (82202-1-RR)

    WB analysis of Jurkat using 82202-1-RR (same clone as 82202-1-PBS)

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Caspase 3 Recombinant antibody (82202-1-RR)

    WB analysis using 82202-1-RR (same clone as 82202-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Caspase 3 Recombinant antibody (82202-1-RR)

    IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Caspase 3 Recombinant antibody (82202-1-RR)

    IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Caspase 3 Recombinant antibody (82202-1-RR)

    IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Caspase 3 Recombinant antibody (82202-1-RR)

    IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Caspase 3 Recombinant antibody (82202-1-RR)

    IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Caspase 3 Recombinant antibody (82202-1-RR)

    IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Caspase 3 Recombinant antibody (82202-1-RR)

    IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Caspase 3 Recombinant antibody (82202-1-RR)

    IF Staining of HeLa using 82202-1-RR (same clone as 82202-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Caspase 3 Recombinant antibody (82202-1-RR)

    IF Staining of HeLa using 82202-1-RR (same clone as 82202-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Caspase 3 Recombinant antibody (82202-1-RR)

    FC experiment of HepG2 using 82202-1-RR (same clone as 82202-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Caspase 3 (82202-1-RR, Clone:5G20) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82202-1-PBS targets Caspase 3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight 32 kDa
Observed molecular weight32-35 kDa, 17 kDa, 19 kDa
GenBank accession numberNM_004346
Gene symbol Caspase 3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.