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  • Featured Product
  • KD/KO Validated

Caspase 6 Monoclonal antibody, PBS Only

Caspase 6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7F9

Cat no : 67825-1-PBS

Synonyms

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Caspase 6 Monoclonal antibody (67825-1-Ig)

    WB analysis using 67825-1-Ig (same clone as 67825-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67825-1-Ig (Caspase 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Caspase 6 Monoclonal antibody (67825-1-Ig)

    WB analysis of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    WB result of Caspase 6 antibody (67825-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6 transfected Jurkat cells. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Caspase 6 Monoclonal antibody (67825-1-Ig)

    IF Staining of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 6 antibody (67825-1-Ig, Clone: 1H7F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67825-1-PBS targets Caspase 6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 6 fusion protein Ag20534 相同性解析による交差性が予測される生物種
Full Name caspase 6, apoptosis-related cysteine peptidase
Calculated molecular weight 33 kDa, 22 kDa
Observed molecular weight 33-35 kDa，22kDa, 18kDa
GenBank accession numberBC000305
Gene symbol Caspase 6
Gene ID (NCBI) 839
RRIDAB_2918587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).