Validation Data Gallery

Filter: AllWBIPIF-PIF/ICC

WB analysis using 27155-1-AP
Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 27155-1-AP
WB result of Caspase 7 antibody (27155-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 7 transfected HEK-293 cells.
WB analysis of HEK-293 using 27155-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 27155-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 27155-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 27155-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 27155-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 27155-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 27155-1-AP
IP result of anti-Caspase 7 (IP:27155-1-AP, 4ug; Detection:27155-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.
IF Staining of mouse brain using 27155-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 27155-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 27155-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 27155-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Caspase 7 antibody (27155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Caspase 7 Polyclonal antibody

Caspase 7 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Cat No. 27155-1-AP

Publications(108)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA, Immunofluorescence, Western Blot

Synonyms

CASP7, Apoptotic protease Mch 3, Apoptotic protease Mch-3, CASP 7, CASP-7

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (108)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, LNCaP cells, PC-3 cells, MCF-7 cells, mouse liver tissue
Positive IP detected in	HEK-293 cells
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 95 publications below
IHC	See 5 publications below
IP	See 1 publications below

Product Information

27155-1-AP targets Caspase 7 in WB, IHC, IF/ICC, IF-P, IP, ELISA, Immunofluorescence, Western Blot applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25904 Product name: Recombinant human Caspase 7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC015799 Sequence: MADEQGCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKC 相同性解析による交差性が予測される生物種
Full Name	caspase 7, apoptosis-related cysteine peptidase
Calculated molecular weight	303 aa, 34 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC015799
Gene Symbol	Caspase 7
Gene ID (NCBI)	840
RRID	AB_2880779
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55210
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

Protocols

Product Specific Protocols
IF protocol for Caspase 7 antibody 27155-1-AP	Download protocol
IP protocol for Caspase 7 antibody 27155-1-AP	Download protocol
WB protocol for Caspase 7 antibody 27155-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human		Adv Mater Selective Organ-Targeting Hafnium Oxide Nanoparticles with Multienzyme-Mimetic Activities Attenuate Radiation-Induced Tissue Damage Authors - Dingxin Liu View Article
		Cell Stem Cell Psychological stress drives aging-like hematopoietic stem cell dysfunction through a brain-gut-bone marrow axis. Authors - Xiaobin Tian View Article
		Autophagy E-cigarette aerosols induce the hydrolysis of lysosomal glycerophospholipids through PLA2G4A activation initiated by nicotine binding to CHRNA3/α3 nAchr in airway epithelial cells. Authors - Yongquan Yu View Article
rat	WB	Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model Authors - Mengyan Hu View Article
chicken	WB	J Adv Res PERK drives aflatoxin B1-induced chicken cellular senescence by promoting NLRP3-mediated PANoptosis. Authors - Yue Cheng View Article
mouse	WB	Gut Microbes Dietary cholesterol impairs cognition via gut microbiota-derived deoxycholic acid in obese mice Authors - Yan Liu View Article

Validation Data Gallery

WB analysis using 27155-1-AP

WB analysis of HEK-293 using 27155-1-AP

WB analysis of HEK-293 using 27155-1-AP

WB analysis of LNCaP using 27155-1-AP

WB analysis of LNCaP using 27155-1-AP

WB analysis of MCF-7 using 27155-1-AP

WB analysis of PC-3 using 27155-1-AP

WB analysis of mouse liver using 27155-1-AP

IP experiment of HEK-293 using 27155-1-AP

IF Staining of mouse brain using 27155-1-AP

IF Staining of mouse brain using 27155-1-AP

IF Staining of mouse brain using 27155-1-AP

IF Staining of MCF-7 using 27155-1-AP

Caspase 7 Polyclonal antibody

Caspase 7 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Selective Organ-Targeting Hafnium Oxide Nanoparticles with Multienzyme-Mimetic Activities Attenuate Radiation-Induced Tissue Damage

Psychological stress drives aging-like hematopoietic stem cell dysfunction through a brain-gut-bone marrow axis.

E-cigarette aerosols induce the hydrolysis of lysosomal glycerophospholipids through PLA2G4A activation initiated by nicotine binding to CHRNA3/α3 nAchr in airway epithelial cells.

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

PERK drives aflatoxin B1-induced chicken cellular senescence by promoting NLRP3-mediated PANoptosis.

Dietary cholesterol impairs cognition via gut microbiota-derived deoxycholic acid in obese mice

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