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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    WB analysis of Jurkat using 66093-1-Ig (same clone as 66093-1-PBS)

    Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8/P43/P18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    WB analysis using 66093-1-Ig (same clone as 66093-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8/P43/P18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    WB analysis of HeLa cells using 66093-1-Ig (same clone as 66093-1-PBS)

    WB result of Caspase 8/P43/P18 with normal and apotosis Hela cell. P18 can be get in the apotosis cell. 60kd,50kd and 45kd bands are some isoforms of precursor CASP8. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    IP experiment of HepG2 using 66093-1-Ig (same clone as 66093-1-PBS)

    IP result of anti-Caspase 8/P43/P18 (IP:66093-1-Ig, 5ug; Detection:66093-1-Ig 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    IHC staining of human liver using 66093-1-Ig (same clone as 66093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    IHC staining of human liver using 66093-1-Ig (same clone as 66093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Caspase 8/P43/P18 Monoclonal antibody (66093-1-Ig)

    IF Staining of HeLa using 66093-1-Ig (same clone as 66093-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 8/P43/P18 antibody (66093-1-Ig, Clone: 2B9H8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Caspase 8/P43/P18 Monoclonal antibody, PBS Only

Caspase 8/P43/P18 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66093-1-PBS
Clone No.2B9H8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CASP8, Caspase 8, 2B9H8, Apoptotic cysteine protease, Apoptotic protease Mch-5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66093-1-PBS targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Caspase 8/P43/P18 fusion protein Ag20524 相同性解析による交差性が予測される生物種
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight 538 aa, 62 kDa
Observed molecular weight 53-57 kDa, 32-45 kDa, 18 kDa
GenBank accession numberBC028223
Gene Symbol Caspase 8
Gene ID (NCBI) 841
RRIDAB_11232214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14790
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.