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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Catalase Monoclonal antibody (66765-1-Ig)

    WB analysis using 66765-1-Ig (same clone as 66765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Catalase Monoclonal antibody (66765-1-Ig)

    WB analysis of HeLa using 66765-1-Ig (same clone as 66765-1-PBS)

    WB result of Catalase antibody (66765-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Catalase transfected HeLa cells. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Catalase Monoclonal antibody (66765-1-Ig)

    WB analysis using 66765-1-Ig (same clone as 66765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Catalase Monoclonal antibody (66765-1-Ig)

    WB analysis of rat brain using 66765-1-Ig (same clone as 66765-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Catalase Monoclonal antibody (66765-1-Ig)

    WB analysis of mouse brain using 66765-1-Ig (same clone as 66765-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Catalase Monoclonal antibody (66765-1-Ig)

    IHC staining of human hepatocirrhosis using 66765-1-Ig (same clone as 66765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Catalase Monoclonal antibody (66765-1-Ig)

    IHC staining of human hepatocirrhosis using 66765-1-Ig (same clone as 66765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Catalase Monoclonal antibody (66765-1-Ig)

    IF Staining of HepG2 using 66765-1-Ig (same clone as 66765-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Catalase antibody (66765-1-Ig, Clone: 1F8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66765-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Catalase Monoclonal antibody, PBS Only

Catalase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66765-1-PBS
Clone No.1F8D7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CAT, EC:1.11.1.6, 1F8D7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66765-1-PBS targets Catalase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Catalase fusion protein Ag16916 相同性解析による交差性が予測される生物種
Full Name catalase
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC112219
Gene Symbol Catalase
Gene ID (NCBI) 847
RRIDAB_2882111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04040
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.