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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cathepsin B Polyclonal antibody

Cathepsin B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31399-1-AP

Synonyms

CTSB, Cathepsin B1, Cathepsin B light chain, Cathepsin B heavy chain, Cat B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin B Polyclonal antibody (31399-1-AP)

    WB analysis using 31399-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 31399-1-AP (Cathepsin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cathepsin B Polyclonal antibody (31399-1-AP)

    IHC staining of human lung cancer using 31399-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 31399-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA375 cells, HepG2 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31399-1-AP targets Cathepsin B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name cathepsin B
Calculated molecular weight38kDa
Observed molecular weight25 kDa and 31 kDa
GenBank accession numberNM_001908.5
Gene symbol Cathepsin B
Gene ID (NCBI) 1508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

Protocols

Product Specific Protocols
WB protocol for Cathepsin B antibody 31399-1-APDownload protocol
IHC protocol for Cathepsin B antibody 31399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols