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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cathepsin D Polyclonal antibody

Cathepsin D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31516-1-AP

Synonyms

CTSD, EC:3.4.23.5, CPSD, CLN10, Cathepsin D light chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin D Polyclonal antibody (31516-1-AP)

    WB analysis using 31516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31516-1-AP (Cathepsin D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Cathepsin D Polyclonal antibody (31516-1-AP)

    IHC staining of human ovary cancer using 31516-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31516-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Cathepsin D Polyclonal antibody (31516-1-AP)

    IHC staining of human ovary cancer using 31516-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31516-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cathepsin D Polyclonal antibody (31516-1-AP)

    IF Staining of A431 using 31516-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cathepsin D antibody (31516-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cathepsin D Polyclonal antibody (31516-1-AP)

    IF Staining of MCF-7 using 31516-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (31516-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HuH-7 cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31516-1-AP targets Cathepsin D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name cathepsin D
Calculated molecular weight45kDa
Observed molecular weight32 kDa, 48 kDa, 52 kDa
GenBank accession numberNM_001909.5
Gene symbol Cathepsin D
Gene ID (NCBI) 1509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSD (cathepsin D) is a lysosomal aspartic protease in the pepsin superfamily ubiquitous to all cell types but particularly abundant in the central nervous system (CNS). The signal peptide of the pre-proCTSD (52 kDa) is cleaved in the endoplasmic reticulum (ER), forming the pro-CTSD (48 kDa), finally processed to form the mature CTSD (32 kDa) in the lysosome (PMID: 31282275,15126630). The major role of the m-CTSD is the digestion of internalized waste cell proteins and peptides in the lysosome, which maintains cell health (PMID: 32324083). CTSD is a well-established aspartyl protease that functions intracellularly in lysosomes and extracellularly in several forms of cancer.

Protocols

Product Specific Protocols
WB protocol for Cathepsin D antibody 31516-1-APDownload protocol
IHC protocol for Cathepsin D antibody 31516-1-APDownload protocol
IF protocol for Cathepsin D antibody 31516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols