• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cathepsin D Recombinant antibody, PBS Only (Detector)

Cathepsin D Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240775E7

Cat no : 83743-2-PBS

Synonyms

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Cathepsin D Recombinant antibody (83743-2-RR)

    WB analysis using 83743-2-RR (same clone as 83743-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Cathepsin D Recombinant antibody (83743-2-RR)

    WB analysis of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00695-4

    Sandwich ELISA standard curve of MP00695-4

    Sandwich ELISA standard curve of MP00695-4, Human Cathepsin D Recombinant Matched Antibody Pair - PBS only. 83743-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1053. 83743-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP00695-4

    Sample test of MP00695-4

    Saliva of eight individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 139.6 ng/mL with a range of 102.4 - 182.3 ng/mL.

  • Sample test of MP00695-4

    Sample test of MP00695-4

    Human milk of eight individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 580.4 ng/mL with a range of 270 - 1,071.8 ng/mL.

  • Sample test of MP00695-4

    Sample test of MP00695-4

    For the A431 supernatant, the mean human Cathepsin D concentration was determined to be 301.05 ng/mL.

  • Sample test of MP00695-4

    Sample test of MP00695-4

    Serum of sixteen individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 147.3 ng/mL with a range of 43.2 - 224.2 ng/mL.

  • Flow cytometry (FC) experiment of MCF-7 cells using Cathepsin D Recombinant antibody (83743-2-RR)

    FC experiment of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cathepsin D Recombinant antibody (83743-2-RR, Clone:240775E7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00695-1

    Cytometric bead array standard curve of MP00695-1

    Cytometric bead array standard curve of MP00695-1, Cathepsin D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83743-1-PBS. Detection antibody: 83743-2-PBS. Standard: Eg1053. Range: 0.78-100 ng/mL.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83743-2-PBS targets Cathepsin D as part of a matched antibody pair:

MP00695-1: 83743-1-PBS capture and 83743-2-PBS detection (validated in Cytometric bead array)

MP00695-4: 83743-6-PBS capture and 83743-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name cathepsin D
Calculated molecular weight45kDa
Observed molecular weight 32 kDa, 48 kDa, 52 kDa
GenBank accession numberNM_001909.5
Gene symbol Cathepsin D
Gene ID (NCBI) 1509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).