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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    WB analysis using 66067-1-Ig (same clone as 66067-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    WB analysis of HeLa using 66067-1-Ig (same clone as 66067-1-PBS)

    WB result of Caveolin-1 antibody (66067-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells. This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IHC staining of human ovary tumor using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IHC staining of human breast cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IHC staining of human breast cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IF Staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IF Staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human skin cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IF Staining of human skin cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human skin cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human skin cancer tissue using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IF Staining of human skin cancer using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human skin cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Caveolin-1 Monoclonal antibody (66067-1-Ig)

    IF Staining of A549 using 66067-1-Ig (same clone as 66067-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Caveolin-1 Monoclonal antibody, PBS Only

Caveolin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66067-1-PBS
Clone No.6C2B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Caveolin 1, CAV1, CGL3, CAV, BSCL3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66067-1-PBS targets Caveolin-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caveolin-1 fusion protein Ag8049 相同性解析による交差性が予測される生物種
Full Name caveolin 1, caveolae protein, 22kDa
Calculated molecular weight 22 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC006432
Gene Symbol CAV1
Gene ID (NCBI) 857
RRIDAB_11043343
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03135
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).