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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Caveolin-1 Recombinant antibody

Caveolin-1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240985E4

Cat no : 84044-1-RR

Synonyms

Caveolin 1, CAV1, CGL3, CAV, BSCL3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Caveolin-1 Recombinant antibody (84044-1-RR)

    WB analysis using 84044-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84044-1-RR (Caveolin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Caveolin-1 Recombinant antibody (84044-1-RR)

    IHC staining of human colon cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using Caveolin-1 Recombinant antibody (84044-1-RR)

    IHC staining of human lung squamous cell carcinoma using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Caveolin-1 Recombinant antibody (84044-1-RR)

    IHC staining of human colon using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84044-1-RR

    Affinity and Kinetic Characterization of 84044-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84044-1-RR against Human Caveolin-1 were performed. The affinity constant is 1.53 nM.

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Tested Applications

Positive WB detected inNIH/3T3 cells, A549 cells, MDA-MB-231 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84044-1-RR is Caveolin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Caveolin-1 fusion protein Ag8049 相同性解析による交差性が予測される生物種
Full Name caveolin 1, caveolae protein, 22kDa
Calculated molecular weight 22 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC006432
Gene symbol CAV1
Gene ID (NCBI) 857
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

Protocols

Product Specific Protocols
WB protocol for Caveolin-1 antibody 84044-1-RRDownload protocol
IHC protocol for Caveolin-1 antibody 84044-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols