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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    WB analysis of mouse colon using 28358-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    WB analysis of mouse heart using 28358-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    WB analysis of mouse skeletal muscle using 28358-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of mouse heart using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of mouse heart using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of rat skeletal muscle using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of rat skeletal muscle using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of mouse skeletal muscle using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of mouse skeletal muscle using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IF Staining of mouse skeletal muscle using 28358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IF Staining of mouse skeletal muscle using 28358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IF Staining of H9C2 using 28358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Caveolin-3 antibody (28358-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Caveolin-3 Polyclonal antibody

Caveolin-3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 28358-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CAV3, Caveolin3, M-caveolin, LQT9, LGMD1C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, mouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Positive IF/ICC detected inH9C2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28358-1-AP targets Caveolin-3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caveolin-3 fusion protein Ag28009 相同性解析による交差性が予測される生物種
Full Name caveolin 3
Calculated molecular weight 151 aa, 17 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC069368
Gene Symbol Caveolin-3
Gene ID (NCBI) 859
RRIDAB_2881120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56539
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Caveolin-3 antibody 28358-1-APDownload protocol
IHC protocol for Caveolin-3 antibody 28358-1-APDownload protocol
IF protocol for Caveolin-3 antibody 28358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Oxid Med Cell Longev

Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor.

Authors - Lin Wang
View Article