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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Caveolin-3 Polyclonal antibody

Caveolin-3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28358-1-AP

Synonyms

CAV3, caveolin 3, Caveolin-3, LGMD1C, LQT9, M caveolin, VIP 21, VIP21

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    WB analysis of mouse colon using 28358-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    WB analysis of mouse heart using 28358-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    WB analysis of mouse skeletal muscle using 28358-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of mouse heart using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of mouse heart using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of mouse skeletal muscle using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of rat skeletal muscle using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of rat skeletal muscle using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IHC staining of mouse skeletal muscle using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IF Staining of mouse skeletal muscle using 28358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Caveolin-3 Polyclonal antibody (28358-1-AP)

    IF Staining of mouse skeletal muscle using 28358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse colon tissue, mouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28358-1-AP targets Caveolin-3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caveolin-3 fusion protein Ag28009 相同性解析による交差性が予測される生物種
Full Name caveolin 3
Calculated molecular weight 151 aa, 17 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC069368
Gene symbol Caveolin-3
Gene ID (NCBI) 859
RRIDAB_2881120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Caveolin-3 antibody 28358-1-APDownload protocol
IHC protocol for Caveolin-3 antibody 28358-1-APDownload protocol
IF protocol for Caveolin-3 antibody 28358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Oxid Med Cell Longev

Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor.

Authors - Lin Wang
View Article