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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCP-1/CCL2 Polyclonal antibody

MCP-1/CCL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31520-1-AP

Synonyms

Ccl2, Chemokine C C motif ligand 2, chemokine (C C motif) ligand 2, CCL-2, CCL 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ccl2 Polyclonal antibody (31520-1-AP)

    WB analysis using 31520-1-AP

    Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 31520-1-AP (Ccl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inLPS and Protein transport inhibitor treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31520-1-AP targets MCP-1/CCL2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 2
Calculated molecular weight16 kDa
Observed molecular weight20 kDa
GenBank accession numberNM_031530
Gene symbol MCP-1/CCL2
Gene ID (NCBI) 24770
RRIDAB_3670017
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Protocols

Product Specific Protocols
WB protocol for MCP-1/CCL2 antibody 31520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols