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  • KD/KO Validated

CD2AP Polyclonal antibody

CD2AP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 51046-1-AP

Synonyms

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein


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Tested Applications

Positive WB detected inmouse kidney tissue, mouse testis tissue, mouse brain tissue, Jurkat cells, A431 cells, HeLa cells
Positive IP detected inmouse brain tissue, HeLa cells
Positive IF/ICC detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51046-1-AP targets CD2AP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD2AP fusion protein Ag0622 相同性解析による交差性が予測される生物種
Full Name CD2-associated protein
Calculated molecular weight 637 aa, 70 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC157901
Gene symbol Cd2ap
Gene ID (NCBI) 12488
RRIDAB_670322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Protocols

Product Specific Protocols
WB protocol for CD2AP antibody 51046-1-APDownload protocol
IF protocol for CD2AP antibody 51046-1-APDownload protocol
IP protocol for CD2AP antibody 51046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Clin Invest

Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function.

Authors - Sei Yoshida
humanWB

Exp Ther Med

Transforming growth factor-β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation.

Authors - Xintong Zheng
humanWB,IF

J Biol Chem

The Rab GTPase-binding protein EHBP1L1 and its interactors CD2AP/CIN85 negatively regulate the length of primary cilia via actin remodeling

Authors - Tomohiko Iwano
  • KD Validated
mouseWB

Int J Mol Sci

The Cleavage-Specific Tau 12A12mAb Exerts an Anti-Amyloidogenic Action by Modulating the Endocytic and Bioenergetic Pathways in Alzheimer's Disease Mouse Model

Authors - Valentina Latina
mouseWB,IF

J Am Soc Nephrol

Glomerulosclerosis Induced by Deficiency of Membrane-Associated Guanylate Kinase Inverted 2 in Kidney Podocytes

Authors - Naritoshi Shirata
humanWB

Int J Cardiol

Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease

Authors - Lili Yu
  • KD Validated