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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    WB analysis of rat liver using 66156-1-Ig (same clone as 66156-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    WB analysis of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    WB analysis of HSC-T6 using 66156-1-Ig (same clone as 66156-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    WB analysis of human kidney using 66156-1-Ig (same clone as 66156-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    WB analysis of human blood using 66156-1-Ig (same clone as 66156-1-PBS)

    human blood was subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IHC staining of human liver using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IHC staining of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IHC staining of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IHC staining of human liver using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ceruloplasmin Monoclonal antibody (66156-1-Ig)

    IF Staining of HeLa using 66156-1-Ig (same clone as 66156-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ceruloplasmin antibody (66156-1-Ig, Clone: 3C8A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

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Ceruloplasmin Monoclonal antibody, PBS Only

Ceruloplasmin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66156-1-PBS
Clone No.3C8A5

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66156-1-PBS targets Ceruloplasmin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ceruloplasmin fusion protein Ag15535 相同性解析による交差性が予測される生物種
Full Name ceruloplasmin (ferroxidase)
Calculated molecular weight 1065 aa, 122 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC146663
Gene Symbol Ceruloplasmin
Gene ID (NCBI) 1356
RRIDAB_2881552
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00450
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.