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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ch-TOG Polyclonal antibody

Ch-TOG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26457-1-AP

Synonyms

CKAP5, ch TOG, XMAP215, TOGp, Cytoskeleton-associated protein 5

Validation Data Gallery

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  • Western Blot (WB) analysis of NCCIT cells using Ch-TOG Polyclonal antibody (26457-1-AP)

    WB analysis of NCCIT using 26457-1-AP

    NCCIT cell were subjected to SDS PAGE followed by western blot with 26457-1-AP (Ch-TOG antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Ch-TOG Polyclonal antibody (26457-1-AP)

    WB analysis using 26457-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26457-1-AP (Ch-TOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Ch-TOG Polyclonal antibody (26457-1-AP)

    WB analysis using 26457-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26457-1-AP (Ch-TOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Ch-TOG Polyclonal antibody (26457-1-AP)

    IHC staining of mouse cerebellum using 26457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26457-1-AP (Ch-TOG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Ch-TOG Polyclonal antibody (26457-1-AP)

    IHC staining of mouse cerebellum using 26457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26457-1-AP (Ch-TOG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Ch-TOG Polyclonal antibody (26457-1-AP)

    IF Staining of HeLa using 26457-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26457-1-AP (Ch-TOG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, NCCIT cells, HepG2 cells, MCF-7 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26457-1-AP targets Ch-TOG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ch-TOG fusion protein Ag24875 相同性解析による交差性が予測される生物種
Full Name cytoskeleton associated protein 5
Observed molecular weight 220 kDa
GenBank accession numberBC120869
Gene symbol Ch-TOG
Gene ID (NCBI) 9793
RRIDAB_2880520
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

Protocols

Product Specific Protocols
WB protocol for Ch-TOG antibody 26457-1-APDownload protocol
IHC protocol for Ch-TOG antibody 26457-1-APDownload protocol
IF protocol for Ch-TOG antibody 26457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Rep

Compartmentalized control of Cdk1 drives mitotic spindle assembly.

Authors - Angela Flavia Serpico
View Article