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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ch-TOG Monoclonal antibody, PBS Only

Ch-TOG Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H5G1

Cat no : 67631-1-PBS

Synonyms

ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ch-TOG Monoclonal antibody (67631-1-Ig)

    WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Ch-TOG Monoclonal antibody (67631-1-Ig)

    WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ch-TOG Monoclonal antibody (67631-1-Ig)

    IF Staining of HepG2 using 67631-1-Ig (same clone as 67631-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (67631-1-Ig, Clone: 1H5G1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67631-1-PBS targets Ch-TOG in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ch-TOG fusion protein Ag28770 相同性解析による交差性が予測される生物種
Full Name cytoskeleton associated protein 5
Observed molecular weight 220-240 kDa
GenBank accession numberBC120869
Gene symbol CKAP5
Gene ID (NCBI) 9793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.