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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ch-TOG Recombinant antibody

Ch-TOG Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241546G8

Cat no : 84285-3-RR

Synonyms

CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ch-TOG Recombinant antibody (84285-3-RR)

    WB analysis using 84285-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84285-3-RR (Ch-TOG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Ch-TOG Recombinant antibody (84285-3-RR)

    IHC staining of mouse cerebellum using 84285-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84285-3-RR (Ch-TOG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Ch-TOG Recombinant antibody (84285-3-RR)

    IHC staining of mouse cerebellum using 84285-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84285-3-RR (Ch-TOG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using Ch-TOG Recombinant antibody (84285-3-RR)

    FC experiment of HeLa using 84285-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ckap5 Recombinant Antibody (84285-3-RR, Clone:241546G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A549 cells using Ch-TOG Recombinant antibody (84285-3-RR)

    FC experiment of A549 using 84285-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Ckap5 Recombinant Antibody (84285-3-RR, Clone:241546G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84285-3-RR

    Affinity and Kinetic Characterization of 84285-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84285-3-RR against Human Ch-TOG were performed. The affinity constant is 1.05 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, NCCIT cells, SH-SY5Y cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84285-3-RR is Ch-TOG Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ch-TOG fusion protein Ag24875 相同性解析による交差性が予測される生物種
Full Name cytoskeleton associated protein 5
Observed molecular weight 220 kDa
GenBank accession numberBC120869
Gene symbol Ch-TOG
Gene ID (NCBI) 9793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

Protocols

Product Specific Protocols
WB protocol for Ch-TOG antibody 84285-3-RRDownload protocol
IHC protocol for Ch-TOG antibody 84285-3-RRDownload protocol
FC protocol for Ch-TOG antibody 84285-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols