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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Chk1 Monoclonal antibody, PBS Only

Chk1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A3A5

Cat no : 60277-1-PBS

Synonyms

CHEK1, Chk1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chk1 Monoclonal antibody (60277-1-Ig)

    WB analysis using 60277-1-Ig (same clone as 60277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Chk1 Monoclonal antibody (60277-1-Ig)

    WB analysis of NIH/3T3 using 60277-1-Ig (same clone as 60277-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Chk1 Monoclonal antibody (60277-1-Ig)

    IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Chk1 Monoclonal antibody (60277-1-Ig)

    IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60277-1-PBS targets Chk1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Chk1 fusion protein Ag0409 相同性解析による交差性が予測される生物種
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight 54 kDa
Observed molecular weight50 kDa
GenBank accession numberBC004202
Gene symbol Chk1
Gene ID (NCBI) 1111
RRIDAB_2881396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.