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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chk1 Recombinant antibody (84905-1-RR)

    WB analysis using 84905-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84905-1-RR (CHK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Chk1 Recombinant antibody (84905-1-RR)

    IF Staining of HeLa using 84905-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHK1 antibody (84905-1-RR, Clone: 240618E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K-562 cells using Chk1 Recombinant antibody (84905-1-RR)

    FC experiment of K-562 using 84905-1-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Chk1 Recombinant antibody (84905-1-RR, Clone:240618E11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Chk1 Recombinant antibody

Chk1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84905-1-RR
Clone No.240618E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

CHEK1, CHK1 checkpoint homolog, Chk 1, CHEK 1, Checkpoint kinase-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, A549 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84905-1-RR targets Chk1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Chk1 fusion protein Ag22993 相同性解析による交差性が予測される生物種
Full Name CHK1 checkpoint homolog (S. pombe)
Calculated molecular weight 54 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC004202
Gene Symbol Chk1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14757
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for Chk1 antibody 84905-1-RRDownload protocol
IF protocol for Chk1 antibody 84905-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols