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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ChromoTek Nano-Secondary® alpaca anti-human IgG, recombinant VHH, for 2x Cys conjugation [CTK0117]

Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fab-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

Applications

IF, WB, FC

Reactivity

Human

Host

Alpaca

Conjugate

2x Cys

Cat no : shuGCys2-2

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Validation Data Gallery

  • The alpaca anti-human IgG Fc-specific recombinant VHH [CTK0117] has a very high affinity (KD <10 pM) for human IgG primary antibody.

    The alpaca anti-human IgG Fc-specific recombinant VHH [CTK0117] has a very high affinity (KD <10 pM) for human IgG primary antibody.

  • The affinity of anti-human IgG recombinant VHH towards human IgG1 was determined using biolayer interferometry (BLI). Biotinylated, monoclonal human IgG1 was immobilized on Streptavidin biosensors and assayed with 1.5 to 25 nM of anti-human IgG recombinant VHH. Fit indicates a 1:1 binding model fitted to the data. 

    The affinity of anti-human IgG recombinant VHH towards human IgG1 was determined using biolayer interferometry (BLI). Biotinylated, monoclonal human IgG1 was immobilized on Streptavidin biosensors and assayed with 1.5 to 25 nM of anti-human IgG recombinant VHH. Fit indicates a 1:1 binding model fitted to the data. 

Product Information

Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fab-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

DescriptionMonovalent, recombinant secondary single domain antibody to human
IgG: alpaca monoclonal Nanobody, Fc-specific, for 2x Cys conjugation
ApplicationsIF, WB, FC
HostAlpaca
ReactivityReactivity: human IgG1, IgG2, IgG3, IgG4, Fc-fragment of human IgG, including wt, PGLALA and PALALA mutations. Slight reactivity to macaque serum. No cross-reactivity: human IgA/IgM/IgE, serum proteins from goat, guinea pig, mouse rabbit, rat, sheep.
Specificity/TargetFc-fragment of human IgG
Conjugate2x Cys
Molecular weight14.5 kDa
Extinction coefficient (280 nm)29700 L·Mol-1·cm-1
TypeSecondary Nanobody
FormatAlpaca single domain antibody, monovalent
Cross-reactivityNo cross-reactivity to human IgA/IgM/IgE, and goat, guinea pig, mouse,
rabbit, rat, sheep serum. Shows slight cross-reactivity to macaque serum.
ImmunogenPurified human IgG
ClonalityMonoclonal Nanobody
CloneCTK0117
RRIDAB_3075306
Affinity (KD)< 10 pM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP, Preservative: 0.09 % Sodium azide
Storage ConditionShipped on dry ice. Store at -80°C. Avoid freeze-thaw cycles. Stable for 1 year at -80°C.
Size500 µg