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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chromogranin B Polyclonal antibody (28663-1-AP)

    WB analysis using 28663-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 28663-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of PC-12 cells using Chromogranin B Polyclonal antibody (28663-1-AP)

    FC experiment of PC-12 using 28663-1-AP

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (28663-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Chromogranin B Polyclonal antibody

Chromogranin B Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 28663-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Synonyms

CHGB, CgB, SCG1, Secretogranin 1, PE-11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-12 cells
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28663-1-AP targets Chromogranin B in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chromogranin B fusion protein Ag29920 相同性解析による交差性が予測される生物種
Full Name chromogranin B (secretogranin 1)
Calculated molecular weight 78 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC000375
Gene Symbol Chromogranin B
Gene ID (NCBI) 1114
RRIDAB_2881188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05060
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Chromogranin B antibody 28663-1-APDownload protocol
FC protocol for Chromogranin B antibody 28663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols