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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Claudin 5 Polyclonal antibody (29767-1-AP)

    WB analysis using 29767-1-AP (same clone as 29767-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Claudin 5 Polyclonal antibody (29767-1-AP)

    IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Claudin 5 Polyclonal antibody (29767-1-AP)

    IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

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Claudin 5 Polyclonal antibody, PBS Only

Claudin 5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29767-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29767-1-PBS targets Claudin 5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 5 fusion protein Ag29044 相同性解析による交差性が予測される生物種
Full Name claudin 5
Calculated molecular weight 218 aa, 23 kDa
Observed molecular weight22 kDa
GenBank accession numberBC032363
Gene Symbol CLDN5
Gene ID (NCBI) 7122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00501
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.