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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Claudin 9 Polyclonal antibody

Claudin 9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30520-1-AP

Synonyms

CLDN9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Claudin 9 Polyclonal antibody (30520-1-AP)

    WB analysis using 30520-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 30520-1-AP (Claudin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Claudin 9 Polyclonal antibody (30520-1-AP)

    IHC staining of human endometrial cancer using 30520-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Claudin 9 Polyclonal antibody (30520-1-AP)

    IHC staining of human endometrial cancer using 30520-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Claudin 9 Polyclonal antibody (30520-1-AP)

    IHC staining of human stomach cancer using 30520-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using Claudin 9 Polyclonal antibody (30520-1-AP)

    IF Staining of T-47D using 30520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Claudin 9 antibody (30520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHuH-7 cells
Positive IHC detected inhuman endometrial cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inT-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30520-1-AP targets Claudin 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 9 fusion protein Ag29607 相同性解析による交差性が予測される生物種
Full Name claudin 9
Calculated molecular weight 217 aa, 23 kDa
Observed molecular weight21 kDa
GenBank accession numberBC051870
Gene symbol Claudin 9
Gene ID (NCBI) 9080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium.They are small (20-27kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.

Protocols

Product Specific Protocols
WB protocol for Claudin 9 antibody 30520-1-APDownload protocol
IHC protocol for Claudin 9 antibody 30520-1-APDownload protocol
IF protocol for Claudin 9 antibody 30520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols