Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IP experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)
IP result of anti-Cleaved Caspase 3/P17/P19 (IP:68773-1-Ig, 4ug; Detection:68773-1-Ig 1:5000) with Staurosporine treated Jurkat cells lysate 720 ug. This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 68773-1-Ig (same clone as 68773-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IHC staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 68773-1-Ig (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IF Staining of mouse liver using 68773-1-Ig (same clone as 68773-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IF Staining of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed 50 μM Cisplatin (18 hours) treated BV-2 cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed 50 μM Cisplatin (18 hours) treated BV-2 cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IF Staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated Jurkat cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated Jurkat cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO. RGAM002 ). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
IF Staining of HSC-T6 using 68773-1-Ig (same clone as 68773-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated HSC-T6 cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed non-treated and 1 μM Staurosporine (1/3/6 hours) treated HSC-T6 cells using Cleaved Caspase 3/P17/P19 antibody (68773-1-Ig, Clone: 2F7B8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)
1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.1 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
×
1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.1 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)
1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
×
1x10^6 non-treated Jurkat cells (dash lines) and staurosporine treated Jurkat cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
FC experiment of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)
1x10^6 non-treated BV-2 cells (dash lines) and cisplatin treated BV-2 cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.
×
1x10^6 non-treated BV-2 cells (dash lines) and cisplatin treated BV-2 cells (solid lines) were intracellularly stained with 0.2 μg Cleaved Caspase 3/P17/P19 Monoclonal antibody (68773-1-Ig, Clone:2F7B8, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 68773-1-PBS in a different storage buffer formulation.

Cleaved Caspase 3/P17/P19 Monoclonal antibody, PBS Only

Cleaved Caspase 3/P17/P19 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Cat No. 68773-1-PBS

Clone No.2F7B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Synonyms

CASP3, Caspase3, Caspase 3, CASP-3, CASP 3

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68773-1-PBS targets Cleaved Caspase 3/P17/P19 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight	32 kDa
Observed molecular weight	17 kDa, 19 kDa
GenBank accession number	NM_032991
Gene Symbol	Caspase 3
Gene ID (NCBI)	836
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P42574
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

WB analysis using 68773-1-Ig (same clone as 68773-1-PBS)

IP experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

IHC staining of human ovary cancer using 68773-1-Ig (same clone as 68773-1-PBS)

IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)

IHC staining of human lymphoma using 68773-1-Ig (same clone as 68773-1-PBS)

IHC staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

IF Staining of mouse liver using 68773-1-Ig (same clone as 68773-1-PBS)

IF Staining of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)

IF Staining of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

IF Staining of HSC-T6 using 68773-1-Ig (same clone as 68773-1-PBS)

FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

FC experiment of Jurkat using 68773-1-Ig (same clone as 68773-1-PBS)

FC experiment of BV-2 using 68773-1-Ig (same clone as 68773-1-PBS)

Cleaved Caspase 3/P17/P19 Monoclonal antibody, PBS Only

Cleaved Caspase 3/P17/P19 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information