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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Clusterin Monoclonal antibody, PBS Only

Clusterin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12G10

Cat no : 66109-1-PBS

Synonyms

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Clusterin Monoclonal antibody (66109-1-Ig)

    WB analysis of human plasma using 66109-1-Ig (same clone as 66109-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66109-1-Ig (Clusterin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66109-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human liver using 66109-1-Ig (same clone as 66109-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66109-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human liver using 66109-1-Ig (same clone as 66109-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66109-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human prostate cancer using 66109-1-Ig (same clone as 66109-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66109-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human breast cancer using 66109-1-Ig (same clone as 66109-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66109-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human breast cancer using 66109-1-Ig (same clone as 66109-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66109-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human prostate cancer using 66109-1-Ig (same clone as 66109-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66109-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66109-1-PBS targets Clusterin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Clusterin fusion protein Ag19236 相同性解析による交差性が予測される生物種
Full Name clusterin
Calculated molecular weight 449 aa, 53 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC010514
Gene symbol CLU
Gene ID (NCBI) 1191
RRIDAB_2881508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).