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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Clusterin Recombinant antibody (84067-4-RR)

    WB analysis of human plasma using 84067-4-RR (same clone as 84067-4-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84067-4-RR (Clusterin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84067-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Clusterin Recombinant antibody (84067-4-RR)

    IF Staining of HeLa using 84067-4-RR (same clone as 84067-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (84067-4-RR, Clone: 241277D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84067-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84067-4-RR

    Affinity and Kinetic Characterization of 84067-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human Clusterin were performed. The affinity constant is 6.91 nM.

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Clusterin Recombinant antibody, PBS Only

Clusterin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84067-4-PBS
Clone No.241277D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84067-4-PBS targets Clusterin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name clusterin
Calculated molecular weight 449 aa, 53 kDa
Observed molecular weight37 kDa
GenBank accession numberBC010514
Gene Symbol CLU
Gene ID (NCBI) 1191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10909
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).