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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Tissue Factor Recombinant antibody

Tissue Factor Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240784H6

Cat no : 83776-4-RR

Synonyms

F3, Coagulation factor III, CD142, TFA, TF

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Tissue Factor Recombinant antibody (83776-4-RR)

    WB analysis of HepG2 using 83776-4-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83776-4-RR (Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Tissue Factor Recombinant antibody (83776-4-RR)

    WB analysis of A431 using 83776-4-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 83776-4-RR (Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Tissue Factor Recombinant antibody (83776-4-RR)

    IHC staining of human stomach cancer using 83776-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83776-4-RR (Tissue Factor antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Tissue Factor Recombinant antibody (83776-4-RR)

    IF Staining of A431 using 83776-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Tissue Factor antibody (83776-4-RR, Clone: 240784H6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83776-4-RR

    Affinity and Kinetic Characterization of 83776-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83776-4-RR against Human Coagulation Factor III/Tissue Factor were performed. The affinity constant is 0.131 nM.

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Tested Applications

Positive WB detected inHepG2 cells, A431 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83776-4-RR is Tissue Factor Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name coagulation factor III (thromboplastin, tissue factor)
Calculated molecular weight33 kDa
Observed molecular weight45 kDa
GenBank accession numberNM_001993.5
Gene symbol F3
Gene ID (NCBI) 2152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

Protocols

Product Specific Protocols
WB protocol for Tissue Factor antibody 83776-4-RRDownload protocol
IHC protocol for Tissue Factor antibody 83776-4-RRDownload protocol
IF protocol for Tissue Factor antibody 83776-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols