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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cofilin Monoclonal antibody (66057-1-Ig)

    WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cofilin Monoclonal antibody (66057-1-Ig)

    WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cofilin Monoclonal antibody (66057-1-Ig)

    WB analysis using 66057-1-Ig (same clone as 66057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Cofilin Monoclonal antibody (66057-1-Ig)

    IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Cofilin Monoclonal antibody (66057-1-Ig)

    IHC staining of human testis using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cofilin Monoclonal antibody (66057-1-Ig)

    IHC staining of human lung cancer using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cofilin Monoclonal antibody (66057-1-Ig)

    IHC staining of human lung cancer using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Cofilin Monoclonal antibody (66057-1-Ig)

    IHC staining of human ovary tumor using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Cofilin Monoclonal antibody (66057-1-Ig)

    IHC staining of human ovary tumor using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cofilin Monoclonal antibody (66057-1-Ig)

    IF Staining of HeLa using 66057-1-Ig (same clone as 66057-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cofilin antibody (66057-1-Ig, Clone: 1G6A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66057-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Cofilin Monoclonal antibody, PBS Only

Cofilin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66057-1-PBS
Clone No.1G6A2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CFL1, Cofilin1, Cofilin, non-muscle isoform, Cofilin 1, 1G6A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66057-1-PBS targets Cofilin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag18044

Product name: Recombinant human Cofilin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-166 aa of BC012318

Sequence: MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

相同性解析による交差性が予測される生物種
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC012318
Gene Symbol Cofilin
Gene ID (NCBI) 1072
RRIDAB_11043339
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23528
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.