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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type I Monoclonal antibody, PBS Only

Collagen Type I Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9A7

Cat no : 67288-1-PBS

Synonyms

COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen

Validation Data Gallery

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  • Western Blot (WB) analysis of pig colon tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    WB analysis of pig colon using 67288-1-Ig (same clone as 67288-1-PBS)

    pig colon tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig lung tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    WB analysis of pig lung using 67288-1-Ig (same clone as 67288-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig skin tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    WB analysis of pig skin using 67288-1-Ig (same clone as 67288-1-PBS)

    pig skin tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    WB analysis of human placenta using 67288-1-Ig (same clone as 67288-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human cervical cancer tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    WB analysis of human cervical cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    human cervical cancer tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    IHC staining of human breast cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    IHC staining of human breast cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    IHC staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    IHC staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    IHC staining of human colon using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    IHC staining of human colon using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    IF Staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Collagen Type I Monoclonal antibody (67288-1-Ig)

    IF Staining of human colon cancer using 67288-1-Ig (same clone as 67288-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67288-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67288-1-PBS targets Collagen Type I in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name collagen, type I, alpha 1
Calculated molecular weight139 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberNM_000088
Gene symbol COL1A1
Gene ID (NCBI) 1277
RRIDAB_2882554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727).