Collagen Type II Polyclonal antibody
Collagen Type II Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
WB, IHC, IF/ICC, IF-P, CoIP, ELISA
Conjugate
Unconjugated
254
Cat no : 28459-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 162 publications below |
| IHC | See 101 publications below |
| IF | See 99 publications below |
| CoIP | See 2 publications below |
Product Information
28459-1-AP targets Collagen Type II in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Collagen Type II fusion protein Ag29265 相同性解析による交差性が予測される生物種 |
| Full Name | collagen, type II, alpha 1 |
| Calculated molecular weight | 134 kDa |
| GenBank accession number | BC007252 |
| Gene symbol | COL2A1 |
| Gene ID (NCBI) | 1280 |
| RRID | AB_2881147 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
| IF protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy | ||
Biomaterials Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration | ||
Nat Commun Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. | ||
J Musculoskelet Neuronal Interact Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway | ||
Acta Biomater The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Balawant (Verified Customer) (12-20-2022) | Ive used this Antibody. this great antibody for IHC.
|
