Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
| Positive IF/ICC detected in | ATDC-5 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 183 publications below |
| IHC | See 117 publications below |
| IF | See 118 publications below |
| CoIP | See 2 publications below |
Product Information
28459-1-AP targets Collagen Type II in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Collagen Type II fusion protein Ag29265 相同性解析による交差性が予測される生物種 |
| Full Name | collagen, type II, alpha 1 |
| Calculated molecular weight | 134 kDa |
| GenBank accession number | BC007252 |
| Gene Symbol | COL2A1 |
| Gene ID (NCBI) | 1280 |
| RRID | AB_2881147 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02458 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues .
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
| IF protocol for Collagen Type II antibody 28459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy | ||
Biomaterials Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration | ||
Nat Commun Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. | ||
J Musculoskelet Neuronal Interact Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway | ||
Acta Biomater The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage. |