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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type IX Polyclonal antibody

Collagen Type IX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24148-1-AP

Synonyms

COL9A2, Collagen alpha 2(IX) chain, Collagen IX, Collagen Type IX, collagen, type IX, alpha 2, EDM2, MED

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type IX Polyclonal antibody (24148-1-AP)

    WB analysis using 24148-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24148-1-AP (Collagen Type IX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse ovary tissue, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24148-1-AP targets Collagen Type IX in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type IX fusion protein Ag21458 相同性解析による交差性が予測される生物種
Full Name collagen, type IX, alpha 2
Calculated molecular weight 689 aa, 65 kDa
Observed molecular weight68 kDa
GenBank accession numberBC136326
Gene symbol COL9A2
Gene ID (NCBI) 1298
RRIDAB_3085726
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. This chain is unusual in that, unlike the other two type IX alpha chains, it contains a covalently attached glycosaminoglycan side chain. Mutations in this gene are associated with multiple epiphyseal dysplasia.

Protocols

Product Specific Protocols
WB protocol for Collagen Type IX antibody 24148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols