• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type V Monoclonal antibody, PBS Only

Collagen Type V Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C2C2

Cat no : 67604-1-PBS

Synonyms

COL5A1, Collagen alpha-1(V) chain, Collagen alpha 1(V) chain, 2C2C2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HT-1080 cells using Collagen Type V Monoclonal antibody (67604-1-Ig)

    WB analysis of HT-1080 using 67604-1-Ig (same clone as 67604-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Collagen Type V Monoclonal antibody (67604-1-Ig)

    WB analysis using 67604-1-Ig (same clone as 67604-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Collagen Type V Monoclonal antibody (67604-1-Ig)

    WB analysis using 67604-1-Ig (same clone as 67604-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using Collagen Type V Monoclonal antibody (67604-1-Ig)

    WB analysis of mouse colon using 67604-1-Ig (same clone as 67604-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Collagen Type V Monoclonal antibody (67604-1-Ig)

    WB analysis of HeLa using 67604-1-Ig (same clone as 67604-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Collagen Type V Monoclonal antibody (67604-1-Ig)

    WB analysis using 67604-1-Ig (same clone as 67604-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Collagen Type V Monoclonal antibody (67604-1-Ig)

    IHC staining of human breast cancer using 67604-1-Ig (same clone as 67604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Collagen Type V Monoclonal antibody (67604-1-Ig)

    IHC staining of human breast cancer using 67604-1-Ig (same clone as 67604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Collagen Type V Monoclonal antibody (67604-1-Ig)

    IF Staining of U2OS using 67604-1-Ig (same clone as 67604-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Collagen Type V antibody (67604-1-Ig, Clone: 2C2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67604-1-PBS targets Collagen Type V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Purified native protein corresponding to human collagen type V 相同性解析による交差性が予測される生物種
Full Name collagen, type V, alpha 1
Calculated molecular weight 184 kDa
Observed molecular weight 220 kDa
GenBank accession numberNM_000093
Gene symbol COL5A1
Gene ID (NCBI) 1289
RRIDAB_2882810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.