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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type VI Monoclonal antibody

Collagen Type VI Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E9C2

Cat no : 68652-1-Ig

Synonyms

COL6A2, Collagen alpha 2(VI) chain, collagen, type VI, alpha 2, DKFZp586E1322, FLJ46862, PP3610

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Collagen Type VI Monoclonal antibody (68652-1-Ig)

    WB analysis of human placenta using 68652-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68652-1-Ig (COL6A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Collagen Type VI Monoclonal antibody (68652-1-Ig)

    WB analysis of human heart using 68652-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 68652-1-Ig (COL6A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman placenta tissue, human heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68652-1-Ig targets Collagen Type VI in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Collagen Type VI fusion protein Ag7134 相同性解析による交差性が予測される生物種
Full Name collagen, type VI, alpha 2
Calculated molecular weight 109 kDa
Observed molecular weight140 kDa
GenBank accession numberBC065509
Gene symbol COL6A2
Gene ID (NCBI) 1292
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collagen VI, composed of three polypeptides, alpha-1(VI), alpha-2(VI), and alpha-3(VI) chains, is a beaded filament collagen found in most connective tissues. The alpha-1(VI) and alpha-2(VI) chains are 140 kDa, while the alpha-3(VI) chain is 200-300 kDa (PMID: 3792302, 3348212). COL6A2 gene encodes the alpha-2 subunit of type VI collagen. The alpha-2(VI) subunit has a calculated molecular weight of 109 kDa, and an apparent molecular weight of 140 kDa due to posttranslational modification. Mutations in COL6A2 gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene.

Protocols

Product Specific Protocols
WB protocol for Collagen Type VI antibody 68652-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols