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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Collagen Type XXVIII Polyclonal antibody

Collagen Type XXVIII Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24401-1-AP

Synonyms

COL28A1, Collagen alpha-1(XXVIII) chain, Collagen alpha 1(XXVIII) chain, COL28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Collagen Type XXVIII Polyclonal antibody (24401-1-AP)

    WB analysis using 24401-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24401-1-AP (Collagen Type XXVIII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Collagen Type XXVIII Polyclonal antibody (24401-1-AP)

    IHC staining of human stomach cancer using 24401-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24401-1-AP (Collagen Type XXVIII antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using Collagen Type XXVIII Polyclonal antibody (24401-1-AP)

    IF Staining of U2OS using 24401-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Collagen Type XXVIII antibody (24401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24401-1-AP targets Collagen Type XXVIII in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type XXVIII fusion protein Ag21459 相同性解析による交差性が予測される生物種
Full Name collagen, type XXVIII, alpha 1
Calculated molecular weight 1125 aa, 117 kDa
Observed molecular weight72 kDa, 100-117 kDa
GenBank accession numberBC136892
Gene symbol COL28A1
Gene ID (NCBI) 340267
RRIDAB_3669435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL28A1 (collagen type XXVIII alpha 1 chain), also known as COL28. It is expected to be located in the basement membranes, cytoplasm and nucleus. And the protein is mainly expressed in salivary gland and adrenal. This antibody can recognize the 117 kDa and 72 kDa isoforms of target. COL28A1 is a new collagen that cannot clearly be assigned to any collagen subgroup, despite the fact that it contains VWA domains. Ongoing investigations will shed further light on the role of COL28A1 in the assembly of nerve fiber basement membranes. Although no human mutation has been associated with COL28A1, it is tempting to speculate that mutations in this gene would lead to neurodegenerative disease (PMID: 16330543).

Protocols

Product Specific Protocols
WB protocol for Collagen Type XXVIII antibody 24401-1-APDownload protocol
IF protocol for Collagen Type XXVIII antibody 24401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols