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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Complement factor B Monoclonal antibody (66154-1-Ig)

    WB analysis of human plasma using 66154-1-Ig (same clone as 66154-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66154-1-Ig (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IHC staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IF Staining of human liver using 66154-1-Ig (same clone as 66154-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66154-1-PBS in a different storage buffer formulation.

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Complement factor B Monoclonal antibody, PBS Only

Complement factor B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66154-1-PBS
Clone No.8D2B6

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66154-1-PBS targets Complement factor B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Complement factor B fusion protein Ag19107 相同性解析による交差性が予測される生物種
Full Name complement factor B
Calculated molecular weight 86 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC007990
Gene Symbol Complement factor B
Gene ID (NCBI) 629
RRIDAB_2881550
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP00751
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.