Validation Data Gallery
Tested Applications
| Positive WB detected in | rat heart tissue, human liver tissue, PC-3 cells, mouse heart tissue |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
16960-1-AP targets Connexin-26 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Connexin-26 fusion protein Ag10666 相同性解析による交差性が予測される生物種 |
| Full Name | gap junction protein, beta 2, 26kDa |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC017048 |
| Gene Symbol | Connexin-26 |
| Gene ID (NCBI) | 2706 |
| RRID | AB_2110782 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29033 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin-26 (GJB2) is a beta class gap junction protein. Mutations in the gene for connexin-26 are responsible for as much as 50% of pre-lingual, recessive deafness.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Connexin-26 antibody 16960-1-AP | Download protocol |
| IF protocol for Connexin-26 antibody 16960-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Res Microenvironment Analysis of Prognosis and Molecular Signature of Immune-Related Genes in Lung Adenocarcinoma. | ||
Mol Med Rep Molecular hydrogen accelerates the reversal of acute obstructive cholangitis‑induced liver dysfunction by restoring gap and tight junctions. |