CoraLite® Plus 488 TUNEL Assay Apoptosis Detection Kit (green)
Cat no : PF00006
Validation Data Gallery
Product Information
A distinctive feature of apoptosis is degradation of DNA. The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis. This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells. CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer. The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.
Components
Components | 20T | 50T |
CL488 TUNEL Reaction Buffer | 1 mL | 2 × 1.25 mL |
TdT Enzyme | 20 uL | 50 uL |
Proteinase K (2 mg/mL) | 40 uL | 100 uL |
DNase I (2 U/μL） | 5 uL | 13 uL |
10 X DNase I Buffer | 100 uL | 260 uL |
Note: Due to the upgrade and optimization of some TUNEL series products, the original component "TUNEL Equilibration Buffer" has been removed from some products, which will not affect the effect of the assay, and the step of incubation with equilibration solution can be directly omitted from the TUNEL reaction procedure.
Storage
Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. Avoid freeze- thaw cycles.
Conjugated
CoraLite® Plus 488 label, CL488 for short, this dye is similar to FITC and other dyes.
Ex/Em: 490/515nm
Publications
| Application | Title |
|---|---|
Pharmacol Res Transfer of miR-877-3p via extracellular vesicles derived from dental pulp stem cells attenuates neuronal apoptosis and facilitates early neurological functional recovery after cerebral ischemia-reperfusion injury through the Bclaf1/p53 signaling pathway | |
Oxid Med Cell Longev ProNGF/NGF Modulates Autophagy and Apoptosis through PI3K/Akt/mTOR and ERK Signaling Pathways following Cerebral Ischemia-Reperfusion in Rats. | |
ACS Chem Neurosci Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine. | |
ACS Biomater Sci Eng siRNA Delivery with Stem Cell Membrane-Coated Magnetic Nanoparticles for Imaging-Guided Photothermal Therapy and Gene Therapy | |
J Cell Mol Med The total xanthones extracted from Gentianella acuta alleviates HFpEF by activating the IRE1α/Xbp1s pathway | |
Sci Rep Effect of scutellarin on BV-2 microglial-mediated apoptosis in PC12 cells via JAK2/STAT3 signalling pathway |