CoraLite®594-Phalloidin (red)

Excitation Laser

593 nm

Emission

614 nm

Application

IF

Cat no : PF00003



Product Information

Phalloidin is a toxin isolated from mushrooms that binds F-Actin in the cytoskeleton. This product is conjugated to our fluorescent CoraLite(R) dye to stain F-actin for labeling, identifying and quantifying actin filaments in immunostaining studies. Compared to many fluorescent dyes, CoraLite(R) dyes have advantages in brightness, light stability and water solubility. Excitation/Emission maxima wavelengths are 593 nm/614 nm

Conjugated

CoraLite®594-Phalloidin(red).

Ex/Em: 593/614 nm

Storage

Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. 

Protocol

Stock preparation: Prepare a 200T/mL stock solution by dissolving lyophilized phalloidin (300T) in 1.5 mL sterile water and protect solution from light.

Fluorescence Microscopy Protocol

Cautions

This product is in the form of freeze-dried powder. Please centrifuge it immediately before use and dissolve it in a suitable solvent before use. Phalloidin is highly toxic, please take protective measures .

Stock preparation

Prepare a 200T/mL stock solution by dissolving lyophilized phalloidin (300T) in 1.5 mL sterile water and protect solution from light.

Publications

ApplicationTitle
IF

Adv Sci (Weinh)

Regulating Type H Vessel Formation and Bone Metabolism via Bone-Targeting Oral Micro/Nano-Hydrogel Microspheres to Prevent Bone Loss

Authors - Junjie Li
IF

Antiviral Res

Host factor DUSP5 potently inhibits dengue virus infection by modulating cytoskeleton rearrangement

Authors - Minqi Liang

Front Cell Dev Biol

SHIP1 Activator AQX-1125 Regulates Osteogenesis and Osteoclastogenesis Through PI3K/Akt and NF-κb Signaling.

Authors - Xudong Xie
IF

Food Chem Toxicol

Co-exposure to polystyrene microplastics and microcystin-LR aggravated male reproductive toxicity in mice

Authors - Hongru Liu

Eur J Med Res

Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing macromolecular crowding in vitro

Authors - Qing Liu
IF

Acta Biochim Biophys Sin (Shanghai)

Methyl 3,4-dihydroxybenzoate inhibits RANKL-induced osteoclastogenesis via Nrf2 signaling in vitro and suppresses LPS-induced osteolysis and ovariectomy-induced osteoporosis in vivo

Authors - Zhaobo Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Angie (Verified Customer) (07-01-2024)

4% PFA-fixed A549 cells were permeabilized with 0.1% Triton X-100 in PBS prior to staining. Cells were incubated with CoraLite®594-conjugated Phalloidin for 30 min at room temperature. The nuclei were counterstained with DAPI.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: A549
FH

Udesh (Verified Customer) (03-15-2024)

Significantly cheaper but equally good alternative to Life Tech's Phalloidin. Worked well at 1:400 in 2% BSA for 1 hour at room temperature

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: hMSCs
FH

Balawant (Verified Customer) (12-25-2023)

Very good antibody and with highly specific. This antibody has no background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: caco2, HCT116 cells