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Purchase

CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L)

Applications

IF, FC

Conjugate

CoraLite®647

Host

Donkey

Reactivity

Mouse

Cat no : SA00014-8

Validation Data Gallery

  • Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66009-1-Ig (Beta Actin antiboody) and CoraLite®647 conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) antibody (SA00014-8) at dilution of 1:150.

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66009-1-Ig (Beta Actin antiboody) and CoraLite®647 conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) antibody (SA00014-8) at dilution of 1:150.

  • 1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-human RBAP48 Monoclonal antibody (66060-1-Ig) and CoraLite®647-Conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-human RBAP48 Monoclonal antibody (66060-1-Ig) and CoraLite®647-Conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Product Information

Concentration 0.3 mg/mL
Applications IF, FC
Host Species Donkey
Species Reactivity Mouse
Physical State Liquid
Suggested Dilution Range 1:100 – 1:500
Purity The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Storage Buffer 0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 7.6.
Preservative0.01% Sodium Azide
Storage Instructions Store at -20°C. Stable for one year after shipment.
FluorophoreCL647 Fluorescent Dye. Amax=642nm, Emax=662nm
Stabilizer5 mg/ml BSA
Absorbance RatioA651/A280 = 4.0
ClonalityPolyclonal

Background

N/A

Publications

Title

Bioact Mater

Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis

Authors - Wei Zhang
View Article

Liver Int

Biglycan promotes hepatic fibrosis through activating heat shock protein 47

Authors - Mengli Yu
View Article