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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CstF-64 Monoclonal antibody

CstF-64 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C5G2

Cat no : 68520-1-Ig

Synonyms

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CstF-64 Monoclonal antibody (68520-1-Ig)

    WB analysis using 68520-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68520-1-Ig (CstF-64 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68520-1-Ig targets CstF-64 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CstF-64 fusion protein Ag33751 相同性解析による交差性が予測される生物種
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Calculated molecular weight 61 kDa
Observed molecular weight64-70 kDa
GenBank accession numberBC017712
Gene symbol CstF-64
Gene ID (NCBI) 1478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Protocols

Product Specific Protocols
WB protocol for CstF-64 antibody 68520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols