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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cullin 5 Polyclonal antibody (31183-1-AP)

    WB analysis using 31183-1-AP (same clone as 31183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Cullin 5 Polyclonal antibody (31183-1-AP)

    WB analysis using 31183-1-AP (same clone as 31183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Cullin 5 Polyclonal antibody (31183-1-AP)

    IHC staining of mouse brain using 31183-1-AP (same clone as 31183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Cullin 5 Polyclonal antibody (31183-1-AP)

    IHC staining of mouse brain using 31183-1-AP (same clone as 31183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

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Cullin 5 Polyclonal antibody, PBS Only

Cullin 5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31183-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31183-1-PBS targets Cullin 5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cullin 5 fusion protein Ag34620 相同性解析による交差性が予測される生物種
Full Name cullin 5
Calculated molecular weight 91 kDa
Observed molecular weight80 kDa, 90 kDa
GenBank accession numberBC063306
Gene Symbol Cullin 5
Gene ID (NCBI) 8065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ93034
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).