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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXL15 Polyclonal antibody (31087-1-AP)

    WB analysis using 31087-1-AP

    Mouse lung tissue were subjected to SDS PAGE followed by western blot with 31087-1-AP (CXL15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using Cxcl15 Polyclonal antibody (31087-1-AP)

    IP experiment of mouse lung using 31087-1-AP

    IP result of anti-Cxcl15 (IP:31087-1-AP, 4ug; Detection:31087-1-AP 1:500) with mouse lung tissue lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Cxcl15 Polyclonal antibody (31087-1-AP)

    IHC staining of mouse lung using 31087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31087-1-AP (Cxcl15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Cxcl15 Polyclonal antibody (31087-1-AP)

    IHC staining of mouse lung using 31087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31087-1-AP (Cxcl15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CXL15 Polyclonal antibody

CXL15 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 31087-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IP, ELISA

Synonyms

Cxcl15, chemokine (C X C motif) ligand 15, C-X-C motif chemokine 15, Cxcl-15, Scyb15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31087-1-AP targets CXL15 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXL15 fusion protein Ag33964 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 15
Calculated molecular weight19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC061138
Gene Symbol Cxcl15
Gene ID (NCBI) 20309
RRIDAB_3669847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9WVL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cxcl15, also known as lungkine or WECHE, is a small cytokine belonging to the CXC chemokine family that has been described in the mouse. High levels of Cxcl15 mRNA were specifically detected in the lung and at lower levels in fetal lung tissue by Northern blot and in situ hybridization, suggesting a potential role for this chemokine during lung development. Moreover, the Cxcl15 protein is secreted into the airway spaces and induces the in vitro and in vivo migration of neutrophils, suggesting that it is involved in lung-specific neutrophil trafficking.

Protocols

Product Specific Protocols
WB protocol for CXL15 antibody 31087-1-APDownload protocol
IHC protocol for CXL15 antibody 31087-1-APDownload protocol
IP protocol for CXL15 antibody 31087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols